Document 3163
 DOCN  M94A3163
 TI    Is there any receptor other than CD4 essential for HIV-1 infection?
 DT    9412
 AU    Furlini G; Re MC; Vignoli M; Ramazzotti E; Monari P; La Placa M;
       Institute of Microbiology, University of Bologna, St. Orsola; Hospital,
       Italy.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):139 (abstract no. PA0175). Unique
       Identifier : AIDSLINE ICA10/94369412
 AB    OBJECTIVE: The present work aimed to elucidate whether human
       hematopoietic progenitor cells show substantial and significant
       difference on the presence of membrane molecules in comparison with
       HIV-1 susceptible cell lines, to verify whether the lack of
       infectability of progenitor cells, despite CD4 presence, may be related
       with the absence of other cofactors, present on susceptible cells.
       METHODS: We screened, by means of cytofluorimetric analysis, the
       presence of several membrane proteins (cellular adhesion molecule as
       ICAM, LECAM, HCAM, VLA and other integrin subfamilies, etc.) on CD34+
       cells isolated from bone marrow (BM-CD34+) of healthy subjects and a
       CD34+ cell line (TF-1) in comparison to PBL and three cell lines (H9,
       Jurkat and A3.01) susceptible to HIV-1 infection. RESULTS: Results
       obtained clearly point out that the presence or the absence of such
       membrane molecules doesn't seem to play a role on the susceptibility and
       lack of susceptibility of all the cells tested. One exception could be
       represented by the CD11a molecule that showed a possible intervention on
       virus entry in sensitive cells. DISCUSSION AND CONCLUSIONS: In this work
       we have shown the lack of significant differences on the distribution of
       the various molecules studied among HIV susceptible and unsuceptible
       cells, with the possible exception of CD11a. Our data lead to the idea
       that other surface protein(s) having such cooperating function in viral
       entry must be taken into consideration.
 DE    Acquired Immunodeficiency Syndrome/*MICROBIOLOGY  Antigens,
       CD/*PHYSIOLOGY  Antigens, CD4/*PHYSIOLOGY  Cell Adhesion
       Molecules/*PHYSIOLOGY  Cell Line  Cell Membrane/IMMUNOLOGY  Comparative
       Study  Flow Cytometry/METHODS  Human  HIV-1/*PHYSIOLOGY
       Integrins/*PHYSIOLOGY  Lymphocytes/*IMMUNOLOGY  Receptors,
       Virus/*PHYSIOLOGY  Reference Values  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).